Engineering

Figure 1: Map of each our Level 1 construct

Figure 2: Map of Level 2 construct

Figure 3: Colony PCR result for pJUMP29-1A

Figure 5: Colony PCR results of pJUMP29-1B

Figure 6: Colony PCR results of pJUMP29-1C and pJUMP29-1D

Figure 7: pJUMP29-1D-TphR Colony PCR results

Figure 8: Colony PCR results of pJUMP29-1D-TphR

Figure 11: Colony PCR results for pJUMP29-1B

Figure 12: Colony PCR results of pJUMP29-1C

Figure 13: Colony PCR results of pJUMP29-1B

Figure 14: Ptph-CIAP and TphC-TpiB-TpiA-TphR-β-lactamase design construct

Figure 15: pJUMP49-2B design construct